Aislamiento y Caracterización in vitro de Actinobacterias de Suelos Costeros Frente a Vibrios Patógenos para ser Utilizados como Probióticos en la Acuicultura

Milagro García-Bernal; Ricardo Medina-Marrero; Bernardo Murillo-Amador; Ángel Isidro Campa-Córdova; Dariel Tovar-Ramírez; José Manuel Mazón-Suástegui

doi:10.28940/terralatinoamericana.v43i.2310

Authors

Milagro García-Bernal Centro de Bioactivos Químicos. Universidad central de Las Villas. Carretera a Camajuaní km 5½, CP-54830. Santa Clara, Villa Clara, Cuba https://orcid.org/0000-0002-3350-7284
Ricardo Medina-Marrero Centro de Bioactivos Químicos. Universidad central de Las Villas. Carretera a Camajuaní km 5½, CP-54830. Santa Clara, Villa Clara, Cuba https://orcid.org/0000-0001-5561-5481
Bernardo Murillo-Amador Centro de Investigaciones Biológicas del Noroeste, S.C.
Ángel Isidro Campa-Córdova Centro de Investigaciones Biológicas del Noroeste, S.C. https://orcid.org/0000-0003-0099-3324
Dariel Tovar-Ramírez Centro de Investigaciones Biológicas del Noroeste, S.C.
José Manuel Mazón-Suástegui Centro de Investigaciones Biológicas del Noroeste, S.C https://orcid.org/0000-0003-4074-1180

DOI:

https://doi.org/10.28940/terralatinoamericana.v43i.2310

Keywords:

marine actinomycetes, antibacterial activity, bioprotective agents

Abstract

The use of probiotic bacteria has global importance in aquaculture and aquaponics because they improve growth rates and enhance the organisms resistance to disease, thus increasing production. This study aimed to isolate and characterize actinomycete strains from marine–coastal soils on both coasts of Mexico and to evaluate them in vitro against marine pathogens to determine their probiotic potential and applicability in aquaculture and aquaponics. Fif ty-four actinomycete strains were isolated, and 14 of them exhibited antibacterial activity against the evaluated Vibrio spp. species [V. alginolyticus (CIAD-CAIM 57), V. parahaemolyticus (ATCC-17802), V. harveyi (CIAD-CAIM 1793), and V. vulnificus (CIAD-CAIM 157)]. Based on the results, strain M4 was selected and characterized according to its morphological and biochemical traits. This strain showed enzymatic activity in vitro, confirming its probiotic potential and applicability in aquaculture; it also exhibited increased activity against the pathogenic vibrios tested. These results suggest that strain M4, isolated from coastal marine soil, has probiotic potential and could serve as an alternative to antibiotics to mitigate pathogen attacks in agroaquaculture production systems.

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Isolation and in vitro Characterization of Actinobacteria from Coastal Soils Against Pathogenic Vibrios for Use as Probiotics in Aquaculture

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